Simple Method for Reduction of Autofluorescence in Fluorescence Microscopy
نویسندگان
چکیده
منابع مشابه
Fluorescence correlation microscopy of cells in the presence of autofluorescence.
Fluorescence correlation microscopy (FCM), the combination of fluorescence correlation spectroscopy (FCS) and digital microscopy (Brock and Jovin, 1998. Cell. Mol. Biol. 44:847-856), has been implemented for measuring molecular diffusion and association in living cells with explicit consideration of autocorrelations arising from autofluorescence. Autofluorescence excited at 532 nm colocalizes w...
متن کاملA Simple Transformation Method in Skewness Reduction
Statistical analysis of non-normal data is usually more complicated than that for normaldistribution. In this paper, a simple root/power transformation technique developed by Niaki, et al [1]is extended to transform right and left skewed distributions to nearly normal. The value of theroot/power is explored such that the skewness of the transformed data becomes almost zero with anacceptable err...
متن کاملDevelopment of a Simple and Sensitive Terbium Sensitized Fluorescence Method for Determination of Epinephrine and Norepinephrine in Serum
A simple, rapid, selective and highly sensitive fluorimetric method for determination of two catecholamines, i.e. norepinephrine (NE) and epinephrine (EP), in serum samples was developed. The method is based on the fluorescence sensitization of terbium (Tb3+) by complexation with both catecholamines in the presence of lanthanum (La3+), as a co-cation, and in a Na...
متن کاملwo postprocessing techniques for the elimination f background autofluorescence for fluorescence lifetime maging microscopy
ksana Berezovska rian B. Bacskai radley T. Hyman arvard Medical School assachusetts General Hospital assGeneral Institute for Neurodegenerative Disorders 14 16th Street Abstract. The analysis of fluorescence lifetime imaging microscopy FLIM data under complex biological conditions can be challenging. Particularly, the presence of short-lived autofluorescent aggregates can confound lifetime meas...
متن کاملReduction of coherent artefacts in super‐resolution fluorescence localisation microscopy
Super-resolution localisation microscopy techniques depend on uniform illumination across the field of view, otherwise the resolution is degraded, resulting in imaging artefacts such as fringes. Lasers are currently the light source of choice for switching fluorophores in PALM/STORM methods due to their high power and narrow bandwidth. However, the high coherence of these sources often creates ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 2002
ISSN: 0022-1554,1551-5044
DOI: 10.1177/002215540205000315